3.- Methodology

The method for the experiment would be to use glass cabins totally isolated from the atmospheric environment. Each cabin should have sealed air inputs, to provide the desired air composition to each group of glass cabins. Cabins will accommodate animals for different species which will be divided in various subgroups.  Each group of animals will live in glass cabins with a specific air composition and tested for life span alterations. Glass cabin air compositions as follows:

Group 1: Carbon Dioxide 1%; Nitrogen 80%; Oxygen 18%; trace elements. 1%.

Group 2: Carbon Dioxide 0.5%; Nitrogen 85%; Oxygen 13.5%; trace elements. 1%  

Group 3: Carbon Dioxide 0.3%; Nitrogen 83%; Oxygen 15.5%; trace elements 1.2%

Cabins from each group of air composition should be divided in the following subgroups:

a) Glass cabins with one individual species.

b) Glass cabins with combined species of animals.

c) Glass cabins with animals living in the cabin from birth to death

d) Glass cabins with animals starting to inhabit the cabin only after their period of growth ended.

e) Glass cabins with animals living in the cabin after completed their half-average life span.

f) Glass cabins with animals living in the cabin after completing 2/3 of their average life span

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